Scientist

Certifications & Licenses

Master's or Ph.D. in Biological Sciences or a related field with a minimum of 2 years of experience in an industrial setting focused on the development of stable cell lines for vaccine and biotherapeutic candidates.

Skills

• Extensive hands-on experience with aseptic technique and mammalian cell culture for generating clonally derived cell lines to produce monoclonal antibodies or vaccine candidates.
• Demonstrated ability to produce and screen hundreds of clonally derived CHO or 293 cell lines for top performers.
• Expertise in designing and constructing plasmids for gene expression using molecular cloning methods.
• Hands-on experience with multiple supporting technologies for cell line engineering, including cell counting, high-throughput transfection, single-cell cloning/deposition, flow cytometry, automated liquid handling systems, and automated small-scale bioreactor systems.
• Ability to independently design experiments and work plans and manage project timelines for multiple complex projects simultaneously.
• Experience preparing technical reports, SOPs, data presentations, and documents needed for technology transfer and regulatory filing.
• Expertise in molecular biology techniques for genetic characterization of cell lines, including RT-PCR and NGS.

Responsibilities

• Plan and execute the generation of stable cell lines for preclinical and clinical material production to advance candidates including monoclonal antibodies, recombinant proteins, and virus like particles.
• Use molecular biology techniques, including RT-PCR and NGS, for genetic characterization of cell lines.
• Perform gene synthesis including oligo synthesis, plasmid design, and construction.
• Independently execute all steps of the process including plasmid construction, stable pool generation, single cell cloning, and top clone selection.
• Support the planning and execution of research experiments in the context of the laboratory's overall research program.
• Draft and verify data for CMC sections of pre-IND and IND documents to ensure successful registration of candidates.
• Design, perform, and troubleshoot experiments and analyze data for both development and deliverable projects.
• Support laboratory staff on research projects and techniques.
• Maintain and support one or more pieces of laboratory instrumentation.
• Maintains laboratory equipment and the training of others in their use.
• Assist in maintaining adequate space and facilities for ongoing and projected research studies.
• Identify, propose, and execute process and procedural changes to improve performance, robustness, productivity, and efficiency by reviewing recent literature and new technologies.
• Evaluate, analyze and interpret research data, communicate results and methods in reports, presentations, and manuscripts.
• Develop and track timelines and experimental plans for multiple projects simultaneously and regularly review records of tasks performed by other team members.
• Coordinate with junior staff to modify research protocols.
• Prepare oral presentations and posters for internal and external groups/conferences.
• Present results of clinical and experimental research at recognized scientific meetings by lectures and exhibits (poster sessions).
• Present research at national scientific meetings and symposia.
• Generate, maintain, and review accurate daily records of experiments and results in a laboratory notebook.
• Prepare and review experimental plans, proposals, reports, SOPs, and production records.
• Provide interpretation of results, guidance, and scientific support to the management team.
• Train staff on the proper usage of equipment.
• Mentor junior level scientists.