Scientist

Responsibilities

• Conduct laboratory-based translational research with a focus on drug development for the treatment of cancer.
• Study cultured human and animal cell lines (some derived from cancer tissue) and analyze human and/or animal tissues with and without cancer.
• Perform sophisticated genetic, biological, and biochemical assays including DNA transfection of cells, monolayer and anchorage-independent growth assays, immunoprecipitation assays, agarose gel electrophoresis, in vivo and in vitro kinase assays, and confocal immune-confocal microscopy of cells.
• Support planning and execution of research experiments aligned with the laboratory's overall research program.
• Initiate, design, and develop new projects or assays to advance cancer research, diagnostics, drugs, and vaccines.
• Serve as an authority on tumor biology and assays performed.
• Interpret results, identify shortcomings or pitfalls, and redirect studies for improved outcomes.
• Identify needs for further investigations and propose changes in research direction based on findings and objectives.
• Support laboratory staff on research projects and techniques.
• Work with scientific staff to define key questions from research projects involving in vitro and in vivo analysis of novel therapeutics' impact on immune cells, cancer cells, and mouse-grown tumors, including mechanisms of cell transformation, molecular targets, and biomarker development.
• Collaborate with scientists, statisticians, and expert staff at NCI, NIH, and extramural institutions to advance projects.
• Maintain laboratory equipment and train others in their use.
• Assist in maintaining adequate space and facilities for research studies.
• Evaluate, analyze, and interpret research data; communicate results via reports, presentations, and manuscripts.
• Attend seminars, pursue additional training, participate in meetings and discussions.
• Make oral presentations at conferences and seminars.
• Prepare data sets, tables, figures, and write first-author papers for peer-reviewed journals.
• Participate in special committees, panels, and colloquia.

Requirements

• Ph.D. in Biology, Life Sciences, or related discipline.
• Minimum of 2 years of experience in a laboratory environment.
• Research experience in immunology and/or molecular biology.
• Expertise in mouse handling procedures (intraperitoneal/intravenous injections, tumor measurements, dissections).
• Expertise in immunologic studies including multi-parameter flow cytometric processing and data analysis.
• Expertise in in-vitro functional assays including cell culture, stimulation, proliferation, and cytokine production.
• Expertise in molecular techniques including DNA/RNA extraction and quantification, gel electrophoresis, western blotting, PCR, and immunoprecipitation.
• Knowledge of confocal microscopy (helpful but optional).
• Ability to learn new techniques and teach them to others.
• Knowledge of proper laboratory techniques, disposal methods, laboratory safety procedures, and universal precautions.
• Ability to work independently on projects.
• Excellent analytical, organizational, interpersonal, and time management skills.
• Strong oral and written communication skills.
• Proficiency in Microsoft Office Suite (Word, PowerPoint, Excel).

Nice-to-Haves

• Experience with confocal microscopy.