Biologist
70 – 74Baltimore, MDOnsite
Summary
Part-time biologist supporting NIH/NIA research on cardiovascular aging. Performs molecular biology techniques, cell culture, immunostaining, data analysis, and lab management in Baltimore, MD.
About the role
Responsibilities
- Perform various molecular biology techniques including RNA sequencing, real-time PCR, Western Blotting, and protein analysis
- Perform immunostaining and produce high quality images using confocal microscopy to locate distribution of key proteins in cardiac myocytes and sinoatrial node cells
- Perform cell culture and adenoviral transfection of cardiac myocytes and sinoatrial node cells
- Perform various bioassays and radioimmunoassays
- Prepare and maintain animal study protocols
- Perform data analysis for physiology and biochemical assays
- Maintain accurate research tracking system and database for all research activities
- Keep accurate records of experiments and results
- Submit weekly summary of research activity to primary investigator
- Prepare, organize and maintain various lab samples, supplies, and equipment
- Operate and perform routine maintenance on laboratory instruments and equipment
- Ensure scientific supplies are kept stocked; obtain quotes and prepare purchase requests
- Write and edit scientific papers
- Attend monthly laboratory information meetings
- Actively participate in weekly laboratory meetings by discussing and presenting data and results
- Collaborate with laboratory scientists and participate in planning and managing components of research projects
- Assist in training students and postdoctoral fellows in various research techniques and maintaining laboratory safety
- Collect data, perform statistical analysis and prepare data/results for presentation and publication
Requirements
- Master’s degree in Biotechnology or a related discipline
- Field of study in Biology
- Experience with histopathologic studies of cardiovascular and cerebrovascular remodeling in aging
- Knowledge of cardiovascular diseases in both humans and animal models
- Proficiency with MS Office suite
Nice-to-Haves
- Experience with confocal microscopy
- Experience with RNA sequencing, real-time PCR, Western Blotting
- Experience with cell culture and adenoviral transfection
- Experience with bioassays and radioimmunoassays
- Experience with animal study protocols
- Experience writing and editing scientific papers
Skills
RNA sequencingreal-time PCRWestern Blottingprotein analysisconfocal microscopyimmunostainingcell cultureadenoviral transfectionbioassaysradioimmunoassaysanimal study protocolsstatistical analysisdata analysisMS Office